RAF1 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesNS5; CRAF; Raf-1; c-Raf
ProductOrderR
Clone#4G4
Entrez GenelD5894
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human RAF1 expressed in E. Coli.
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
198-407
Catalog#: 30325
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Images
Western Blot
Figure 1: Western blot analysis using RAF1 mAb against human RAF1 (AA: 198-407) recombinant protein. (Expected MW is 73 kDa)
Figure 1: Western blot analysis using RAF1 mAb against human RAF1 (AA: 198-407) recombinant protein. (Expected MW is 73 kDa)
Western Blot
Figure 2: Western blot analysis using RAF1 mouse mAb against Hela (1), A431 (2), Cos7 (3) and C6 (4) cell lysate.
Figure 2: Western blot analysis using RAF1 mouse mAb against Hela (1), A431 (2), Cos7 (3) and C6 (4) cell lysate.
Immunohistochemical analysis
Figure 3: Immunohistochemical analysis of paraffin-embedded liver cancer tissues using RAF1 mouse mAb with DAB staining.
Figure 3: Immunohistochemical analysis of paraffin-embedded liver cancer tissues using RAF1 mouse mAb with DAB staining.
Flow cytometric
Figure 4: Flow cytometric analysis of Hela cells using RAF1 mouse mAb (green) and negative control (red).
Figure 4: Flow cytometric analysis of Hela cells using RAF1 mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Product Overview
Description

This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.

References (references)
References (references)1. Am J Hum Genet. 2009 Nov;85(5):628-42.
2. Mol Cancer Res. 2009 Oct;7(10):1635-44.