RAD52 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesN
ProductOrderR
Clone#5E11E7
Entrez GenelD5893
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human RAD52 (AA: 269-418) expressed in E. Coli.
MW46.2kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
269-418
Catalog#: 30897
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Images
Elisa
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western Blot
Figure 2:Western blot analysis using RAD52 mAb against human RAD52 (AA: 269-418) recombinant protein. (Expected MW is 42.5 kDa)
Figure 2:Western blot analysis using RAD52 mAb against human RAD52 (AA: 269-418) recombinant protein. (Expected MW is 42.5 kDa)
Western Blot
Figure 3:Western blot analysis using RAD52 mAb against HEK293 (1) and RAD52 (AA: 269-418)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using RAD52 mAb against HEK293 (1) and RAD52 (AA: 269-418)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using RAD52 mouse mAb against HepG2 (1), MCF-7 (2), MCF-7 (3), and C6 (4) cell lysate.
Figure 4:Western blot analysis using RAD52 mouse mAb against HepG2 (1), MCF-7 (2), MCF-7 (3), and C6 (4) cell lysate.
Immunofluorescence analysis
Figure 5:Immunofluorescence analysis of HeLa cells using RAD52 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 5:Immunofluorescence analysis of HeLa cells using RAD52 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 6:Flow cytometric analysis of MCF7 cells using RAD52 mouse mAb (green) and negative control (red).
Figure 6:Flow cytometric analysis of MCF7 cells using RAD52 mouse mAb (green) and negative control (red).
Product Overview
DescriptionThe protein encoded by this gene shares similarity with Saccharomyces cerevisiae Rad52, a protein important for DNA double-strand break repair and homologous recombination. This gene product was shown to bind single-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. It was also found to interact with DNA recombination protein RAD51, which suggested its role in RAD51 related DNA recombination and repair. A pseudogene of this gene is present on chromosome 2. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known.
References (references)
References (references)1.Blood. 2013 Aug 15;122(7):1293-304.
2.PLoS One. 2012;7(11):e50461.