RAB4A Primary Antibody
Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene belongs to the rab family. Rab4A protein is involved in the regulation of procathepsin L secretion, it was either inhibited by expression of a Rab4A dominant-negative mutant or increased by overexpression of the wild-type Rab4A in human melanoma cells.
Product Overview
Entrez GenelD
5867
Aliases
RAB4; HRES-1/RAB4
Clone#
4E11
Host / Isotype
Mouse / IgG1
Species Reactivity
Human, Mouse, Monkey
Immunogen
Purified recombinant fragment of human RAB4A expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1. Biochem J. 2011 Jul 1;437(1):97-107.
2. Int J Cancer. 2011 Jan 1;128(1):192-205.
2. Int J Cancer. 2011 Jan 1;128(1):192-205.
Product Image
Western Blot
Figure 1: Western blot analysis using RAB4A mAb against human RAB4A (AA: 22-218) recombinant protein. (Expected MW is 47.9 kDa)
Western Blot
Figure 2: Western blot analysis using RAB4A mouse mAb against Jurkat (1), HeLa (2), A549 (3), HEK293 (4), K562 (5), NIH3T3 (6), PC-12 (7), and COS7 (8) cell lysate.
Immunohistochemical analysis
Figure 3: Immunohistochemical analysis of paraffin-embedded lung cancer tissues using RAB4A mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 4: Immunohistochemical analysis of paraffin-embedded liver cancer tissues using RAB4A mouse mAb with DAB staining.
Flow cytometric
Figure 5: Flow cytometric analysis of MCF-7 cells using RAB4A mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.