PYCARD Primary Antibody
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Description
This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene.
Product Overview
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Image
Elisa

Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot

Figure 2:Western blot analysis using PYCARD mAb against human PYCARD (AA: 1-120) recombinant protein. (Expected MW is 39.2 kDa)
Western Blot

Figure 3:Western blot analysis using PYCARD mAb against HEK293 (1) and PYCARD (AA: 1-120)-hIgGFc transfected HEK293 (2) cell lysate.
Immunofluorescence analysis

Figure 4:Immunofluorescence analysis of GC-7901 cells using PYCARD mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Immunofluorescence analysis

Figure 5:Immunofluorescence analysis of Hela cells using PYCARD mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric

Figure 6:Flow cytometric analysis of HEK293 cells using PYCARD mouse mAb (green) and negative control (red).
Immunohistochemical analysis

Figure 7:Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using PYCARD mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.