PTPRC Primary Antibody
Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported.
Product Overview
Entrez GenelD
5788
Aliases
LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180
Clone#
5D3A3
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human PTPRC (AA: 928-989) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ELISA
1/10000
References
1. Biochem Biophys Res Commun. 2012 Mar 23;419(4):708-14.
2. Blood. 2009 Jun 4;113(23):5905-10.
2. Blood. 2009 Jun 4;113(23):5905-10.
Product Image
Western Blot
Figure 1: Western blot analysis using PTPRC mAb against human PTPRC (AA: 928-989) recombinant protein. (Expected MW is 33 kDa)
Western Blot
Figure 2: Western blot analysis using PTPRC mAb against HEK293 (1) and PTPRC (AA: 928-989)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemical analysis
Figure 3: Immunohistochemical analysis of paraffin-embedded breast cancer tissues using PTPRC mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 4: Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using PTPRC mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.