PTPN11 Primary Antibody

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Description

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains two tandem Src homology-2 domains, which function as phospho-tyrosine binding domains and mediate the interaction of this PTP with its substrates. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration. Mutations in this gene are a cause of Noonan syndrome as well as acute myeloid leukemia. Two transcript variants encoding different isoforms have been found for this gene.

Product Overview
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
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Western Blot
Figure 1: Western blot analysis using PTPN11 mAb against human PTPN11 recombinant protein. (Expected MW is 33.4 kDa)
Figure 1: Western blot analysis using PTPN11 mAb against human PTPN11 recombinant protein. (Expected MW is 33.4 kDa)
Immunofluorescence analysis
Figure 2: Immunofluorescence analysis of HeLa cells using PTPN11 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Figure 2: Immunofluorescence analysis of HeLa cells using PTPN11 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Flow cytometric
Figure 3: Flow cytometric analysis of HepG2 cells using PTPN11 mouse mAb (green) and negative control (red).
Figure 3: Flow cytometric analysis of HepG2 cells using PTPN11 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 4: Immunohistochemical analysis of paraffin-embedded breast cancer tissues using PTPN11 mouse mAb with DAB staining.
Figure 4: Immunohistochemical analysis of paraffin-embedded breast cancer tissues using PTPN11 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 5: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using PTPN11 mouse mAb with DAB staining.
Figure 5: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using PTPN11 mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.