PTEN Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesBZS; MHAM; TEP1; PTEN1
ProductOrderP
Clone#1B8
Entrez GenelD5728
FormulationAscitic fluid containing 0.03% sodium azide.
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of PTEN expressed in E. Coli.
MW54kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman, Mouse
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
full(1-403)
Catalog#: 20399
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Images
Western Blot
Figure 1: Western blot analysis using PTEN mouse mAb against Hela (1) and NIH/3T3 (2) cell lysate.
Figure 1: Western blot analysis using PTEN mouse mAb against Hela (1) and NIH/3T3 (2) cell lysate.
Immunofluorescence analysis
Figure 2: Confocal Immunofluorescence analysis of Hela (left) and HepG2 (right) cells using PTEN mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Figure 2: Confocal Immunofluorescence analysis of Hela (left) and HepG2 (right) cells using PTEN mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Flow cytometric
Figure 3: Flow cytometric analysis of Hela cells using PTEN mouse mAb (right) and negative control (left).
Figure 3: Flow cytometric analysis of Hela cells using PTEN mouse mAb (right) and negative control (left).
Product Overview
Description

PTEN (phosphatase and tensin homolog) was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. This protein is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway.

References (references)
References (references)1. Nat Genet. 1998 Aug;19(4):348-55.
2. Oncogene. 1999 May 20;18(20):3181-5.
3. Eur J Immunol. 2002 Apr;32(4):1196-204.