PTEN Primary Antibody
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Description
PTEN (phosphatase and tensin homolog) was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. This protein is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway.
Product Overview
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
4°C; -20°C for long term storage
Product Image
Western Blot
Figure 1: Western blot analysis using PTEN mouse mAb against Hela (1) and NIH/3T3 (2) cell lysate.
Immunofluorescence analysis
Figure 2: Confocal Immunofluorescence analysis of Hela (left) and HepG2 (right) cells using PTEN mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Flow cytometric
Figure 3: Flow cytometric analysis of Hela cells using PTEN mouse mAb (right) and negative control (left).
For Research Use Only. Not for use in diagnostic procedures.