PTEN Primary Antibody
Item Information
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Price
Description
PTEN (phosphatase and tensin homolog) was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. This protein is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway.
Product Overview
Entrez GenelD
5728
Aliases
BZS; MHAM; TEP1; PTEN1
Clone#
1B8
Host / Isotype
Mouse / IgG1
Species Reactivity
Human, Mouse
Immunogen
Purified recombinant fragment of PTEN expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1. Nat Genet. 1998 Aug;19(4):348-55.
2. Oncogene. 1999 May 20;18(20):3181-5.
3. Eur J Immunol. 2002 Apr;32(4):1196-204.
2. Oncogene. 1999 May 20;18(20):3181-5.
3. Eur J Immunol. 2002 Apr;32(4):1196-204.
Product Image
Western Blot
Figure 1: Western blot analysis using PTEN mouse mAb against Hela (1) and NIH/3T3 (2) cell lysate.
Immunofluorescence analysis
Figure 2: Confocal Immunofluorescence analysis of Hela (left) and HepG2 (right) cells using PTEN mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Flow cytometric
Figure 3: Flow cytometric analysis of Hela cells using PTEN mouse mAb (right) and negative control (left).
For Research Use Only. Not for use in diagnostic procedures.