PSAP Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesGLBA; SAP1
ProductOrderP
Clone#4D5F4
Entrez GenelD5660
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human PSAP (AA: 325-524 ) expressed in E. Coli.
MW58.1kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
ICC (Immunocytochemistry)1/50 - 1/500
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
325-524
Catalog#: 30444
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Images
Western Blot
Figure 1: Western blot analysis using PSAP mAb against human PSAP recombinant protein. (Expected MW is 47.8 kDa)
Figure 1: Western blot analysis using PSAP mAb against human PSAP recombinant protein. (Expected MW is 47.8 kDa)
Western Blot
Figure 2: Western blot analysis using PSAP mAb against HEK293 (1) and PSAP (AA: 325-524)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 2: Western blot analysis using PSAP mAb against HEK293 (1) and PSAP (AA: 325-524)-hIgGFc transfected HEK293 (2) cell lysate.
Immunofluorescence analysis
Figure 3: Immunofluorescence analysis of HepG2 cells using PSAP mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Figure 3: Immunofluorescence analysis of HepG2 cells using PSAP mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric
Figure 4: Flow cytometric analysis of HeLa cells using PSAP mouse mAb (green) and negative control (purple).
Figure 4: Flow cytometric analysis of HeLa cells using PSAP mouse mAb (green) and negative control (purple).
Immunohistochemical analysis
Figure 5: mmunohistochemical analysis of paraffin-embedded pancreas tissues using PSAP mouse mAb with DAB staining.
Figure 5: mmunohistochemical analysis of paraffin-embedded pancreas tissues using PSAP mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Product Overview
DescriptionThis gene encodes a highly conserved glycoprotein which is a precursor for 4 cleavage products: saposins A, B, C, and D. Each domain of the precursor protein is approximately 80 amino acid residues long with nearly identical placement of cysteine residues and glycosylation sites. Saposins A-D localize primarily to the lysosomal compartment where they facilitate the catabolism of glycosphingolipids with short oligosaccharide groups. The precursor protein exists both as a secretory protein and as an integral membrane protein and has neurotrophic activities. Mutations in this gene have been associated with Gaucher disease, Tay-Sachs disease, and metachromatic leukodystrophy. Alternative splicing results in multiple transcript variants encoding different isoforms.

References (references)
References (references)1.Biochem J. 2012 Jan 15;441(2):665-74.
2.Mol Cancer. 2010 Feb 4;9:30.