PSAP Primary Antibody
|Formulation||Purified antibody in PBS with 0.05% sodium azide|
|Immunogen||Purified recombinant fragment of human PSAP (AA: 325-524 ) expressed in E. Coli.|
|Shipping Information||This product will ship in a box containing blue ice at a temperature of 4°C. Learn More|
|ICC (Immunocytochemistry)||1/50 - 1/500|
|IHC_P(Immunohistochemistry)||1/200 - 1/1000|
|FCM (Flow Cytometry)||1/200 - 1/400|
|WB (Western Blot)||1/500 - 1/2000|
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Figure 1: Western blot analysis using PSAP mAb against human PSAP recombinant protein. (Expected MW is 47.8 kDa)
Figure 2: Western blot analysis using PSAP mAb against HEK293 (1) and PSAP (AA: 325-524)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3: Immunofluorescence analysis of HepG2 cells using PSAP mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Figure 4: Flow cytometric analysis of HeLa cells using PSAP mouse mAb (green) and negative control (purple).
Figure 5: mmunohistochemical analysis of paraffin-embedded pancreas tissues using PSAP mouse mAb with DAB staining.
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
|Description||This gene encodes a highly conserved glycoprotein which is a precursor for 4 cleavage products: saposins A, B, C, and D. Each domain of the precursor protein is approximately 80 amino acid residues long with nearly identical placement of cysteine residues and glycosylation sites. Saposins A-D localize primarily to the lysosomal compartment where they facilitate the catabolism of glycosphingolipids with short oligosaccharide groups. The precursor protein exists both as a secretory protein and as an integral membrane protein and has neurotrophic activities. Mutations in this gene have been associated with Gaucher disease, Tay-Sachs disease, and metachromatic leukodystrophy. Alternative splicing results in multiple transcript variants encoding different isoforms. |
|References (references)||1.Biochem J. 2012 Jan 15;441(2):665-74. |
2.Mol Cancer. 2010 Feb 4;9:30.