PROM1 Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene.
Product Overview
Entrez GenelD
8842
Aliases
RP41; AC133; CD133; MCDR2; STGD4; CORD12; PROML1; MSTP061
Clone#
2F8C5
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human PROM1 (AA: 20-108) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Gastric Cancer. 2014 Jan;17(1):97-106.
2.Cancer Biomark. 2012;12(2):97-105.
2.Cancer Biomark. 2012;12(2):97-105.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western Blot
Figure 2:Western blot analysis using PROM1 mAb against human PROM1 (AA: 20-108) recombinant protein. (Expected MW is 36.1 kDa)
Western Blot
Figure 3:Western blot analysis using PROM1 mAb against HEK293 (1) and PROM1 (AA: 20-108)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of Hela cells using PROM1 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.