PRKDC Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesHYRC; p350; DNAPK; DNPK1; HYRC1; XRCC7; DNA-PKcs; PRKDC
ProductOrderP
Clone#3H6
Entrez GenelD5591
FormulationAscitic fluid containing 0.03% sodium azide.
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human PRKDC expressed in E. Coli.
MW46.9kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
IHC_P(Immunohistochemistry)1/200 - 1/1000
WB (Western Blot)1/500 - 1/2000
Sequence
2638-2971
Catalog#: 30170
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510-740-3625
info@promab.com
Images
Western Blot
Figure 1: Western blot analysis using PRKDC mAb against HEK293 (1) and PRKDC(AA: 2638-2971)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 1: Western blot analysis using PRKDC mAb against HEK293 (1) and PRKDC(AA: 2638-2971)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemical analysis
Figure 2: Immunohistochemical analysis of paraffin-embedded breast cancer (left) and colon cancer (right) using PRKDC mouse mAb with DAB staining.
Figure 2: Immunohistochemical analysis of paraffin-embedded breast cancer (left) and colon cancer (right) using PRKDC mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 3: Immunohistochemical analysis of paraffin-embedded lung cancer (left) and brain tissues (right) using PRKDC mouse mAb with DAB staining.
Figure 3: Immunohistochemical analysis of paraffin-embedded lung cancer (left) and brain tissues (right) using PRKDC mouse mAb with DAB staining.
Immunofluorescence analysis
Figure 4: Immunofluorescence analysis of Hela cells using PRKDC mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Figure 4: Immunofluorescence analysis of Hela cells using PRKDC mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Elisa
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Product Overview
Description

The PRKDC gene encodes the catalytic subunit of a nuclear DNA-dependent serine/threonine protein kinase (DNA-PK). The second component is the autoimmune antigen Ku (MIM 152690), which is encoded by the G22P1 gene on chromosome 22q. On its own, the catalytic subunit of DNA-PK is inactive and relies on the G22P1 component to direct it to the DNA and trigger its kinase activity; PRKDC must be bound to DNA to express its catalytic properties.

References (references)
References (references)1. J Biol Chem. 2008 Dec 26;283(52):36311-20.
2. Proc Natl Acad Sci U S A. 2008 Sep 2;105(35):12791-6.
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