PRKDC Primary Antibody

Item Information

Catalog #

Size

Price

Description

The PRKDC gene encodes the catalytic subunit of a nuclear DNA-dependent serine/threonine protein kinase (DNA-PK). The second component is the autoimmune antigen Ku (MIM 152690), which is encoded by the G22P1 gene on chromosome 22q. On its own, the catalytic subunit of DNA-PK is inactive and relies on the G22P1 component to direct it to the DNA and trigger its kinase activity; PRKDC must be bound to DNA to express its catalytic properties.

Product Overview

Entrez GenelD

5591

Aliases

HYRC; p350; DNAPK; DNPK1; HYRC1; XRCC7; DNA-PKcs; PRKDC

Clone#

3H6

Host / Isotype

Mouse / IgG1

Species Reactivity

Human

Immunogen

Purified recombinant fragment of human PRKDC expressed in E. Coli.

Formulation

Ascitic fluid containing 0.03% sodium azide.

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

ICC (Immunocytochemistry)

1/200 - 1/1000

ELISA

1/10000

References

1. J Biol Chem. 2008 Dec 26;283(52):36311-20.
2. Proc Natl Acad Sci U S A. 2008 Sep 2;105(35):12791-6.

Product Image

Western Blot

Figure 1: Western blot analysis using PRKDC mAb against HEK293 (1) and PRKDC(AA: 2638-2971)-hIgGFc transfected HEK293 (2) cell lysate.

Immunohistochemical analysis

Figure 2: Immunohistochemical analysis of paraffin-embedded breast cancer (left) and colon cancer (right) using PRKDC mouse mAb with DAB staining.

Immunohistochemical analysis

Figure 3: Immunohistochemical analysis of paraffin-embedded lung cancer (left) and brain tissues (right) using PRKDC mouse mAb with DAB staining.

Immunofluorescence analysis

Figure 4: Immunofluorescence analysis of Hela cells using PRKDC mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Elisa