PRKACA Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesPKACA
ProductOrderP
Clone#7H3A4
Entrez GenelD5566
FormulationPurified antibody in PBS with 0.05% sodium azide.
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human PRKACA (AA: 1-120) expressed in E. Coli.
MW40.6kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
WB (Western Blot)1/500 - 1/2000
Sequence
1-120
Catalog#: 30676
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Images
Western Blot
Figure 1: Western blot analysis using PRKACA mAb against human PRKACA (AA: 1-120) recombinant protein. (Expected MW is 39.7 kDa)
Figure 1: Western blot analysis using PRKACA mAb against human PRKACA (AA: 1-120) recombinant protein. (Expected MW is 39.7 kDa)
Western Blot
Figure 2: Western blot analysis using PRKACA mAb against HEK293 (1) and PRKACA (AA: 1-120)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 2: Western blot analysis using PRKACA mAb against HEK293 (1) and PRKACA (AA: 1-120)-hIgGFc transfected HEK293 (2) cell lysate.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Product Overview
Description

cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is a member of the Ser/Thr protein kinase family and is a catalytic subunit of cAMP-dependent protein kinase. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

References (references)
References (references)1. J Struct Biol. 2012 Jun;178(3):300-10.
2. BMC Biochem. 2011 Aug 3;12:40.