PRKAA1 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesAMPK; AMPKa1; MGC33776; MGC57364; PRKAA1
ProductOrderP
Clone#2B7
Entrez GenelD5562
FormulationAscitic fluid containing 0.03% sodium azide.
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human PRKAA1 expressed in E. Coli.
MW64kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman, Monkey, Mouse, Rat
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
357-524
Catalog#: 30172
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Images
Western Blot
Figure 1: Western blot analysis using PRKAA1 mouse mAb against Jurkat (1), Hela (2), HepG2 (3), MCF-7 (4), Cos7 (5), NIH/3T3 (6), K562 (7), HEK293 (8), and PC-12 (9) cell lysate.
Figure 1: Western blot analysis using PRKAA1 mouse mAb against Jurkat (1), Hela (2), HepG2 (3), MCF-7 (4), Cos7 (5), NIH/3T3 (6), K562 (7), HEK293 (8), and PC-12 (9) cell lysate.
Immunohistochemical analysis
Figure 2: Immunohistochemical analysis of paraffin-embedded ovarian cancer (left) and brain tissues (right) using PRKAA1 mouse mAb with DAB staining.
Figure 2: Immunohistochemical analysis of paraffin-embedded ovarian cancer (left) and brain tissues (right) using PRKAA1 mouse mAb with DAB staining.
Immunofluorescence analysis
Figure 3: Immunofluorescence analysis of NTERA-2 cells using PRKAA1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Figure 3: Immunofluorescence analysis of NTERA-2 cells using PRKAA1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric
Figure 4: Flow cytometric analysis of PC-2 cells using PRKAA1 mouse mAb (green) and negative control (purple).
Figure 4: Flow cytometric analysis of PC-2 cells using PRKAA1 mouse mAb (green) and negative control (purple).
Elisa
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Product Overview
Description

The protein belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

References (references)
References (references)1. Oncol Rep. 2008 Dec;20(6):1553-9.
2. Placenta. 2008 Dec;29(12):1003-8.