PRKAA1 Primary Antibody
| Item Information | ||
|---|---|---|
| Catalog # | Size/Concentration | Price |
| Specification | |
|---|---|
| Aliases | AMPK; AMPKa1; MGC33776; MGC57364; PRKAA1 |
| ProductOrder | P |
| Clone# | 2B7 |
| Entrez GenelD | 5562 |
| Formulation | Ascitic fluid containing 0.03% sodium azide. |
| Host | Mouse |
| Isotype | IgG1 |
| Immunogen | Purified recombinant fragment of human PRKAA1 expressed in E. Coli. |
| MW | 64kDa |
| Shipping Information | This product will ship in a box containing blue ice at a temperature of 4°C. Learn More |
| Species Reactivity | Human, Monkey, Mouse, Rat |
| Application | |
|---|---|
| ELISA | 1/10000 |
| ICC (Immunocytochemistry) | 1/200 - 1/1000 |
| IHC_P(Immunohistochemistry) | 1/200 - 1/1000 |
| FCM (Flow Cytometry) | 1/200 - 1/400 |
| WB (Western Blot) | 1/500 - 1/2000 |
| Sequence | |
|---|---|
| 357-524 | |
Western Blot
Figure 1: Western blot analysis using PRKAA1 mouse mAb against Jurkat (1), Hela (2), HepG2 (3), MCF-7 (4), Cos7 (5), NIH/3T3 (6), K562 (7), HEK293 (8), and PC-12 (9) cell lysate.
Immunohistochemical analysis
Figure 2: Immunohistochemical analysis of paraffin-embedded ovarian cancer (left) and brain tissues (right) using PRKAA1 mouse mAb with DAB staining.
Immunofluorescence analysis
Figure 3: Immunofluorescence analysis of NTERA-2 cells using PRKAA1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric
Figure 4: Flow cytometric analysis of PC-2 cells using PRKAA1 mouse mAb (green) and negative control (purple).
Elisa
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);