PRK2 Primary Antibody
|Aliases||PKN2; PAK2; PRK2; Pak-2; PRKCL2; PRO2042; MGC71074; MGC150606|
|Formulation||Ascitic fluid containing 0.03% sodium azide.|
|Immunogen||Purified recombinant fragment of human PRK2 expressed in E. Coli. |
|Shipping Information||This product will ship in a box containing blue ice at a temperature of 4°C. Learn More|
|Species Reactivity||Human, Monkey, Mouse, Rat|
|IHC_P(Immunohistochemistry)||1/200 - 1/1000|
|FCM (Flow Cytometry)||1/200 - 1/400|
|WB (Western Blot)||1/500 - 1/2000|
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Figure 1: Western blot analysis using PRK2 mAb against human PRK2 (AA: 555-718) recombinant protein. (Expected MW is 43.9 kDa)
Figure 2: Western blot analysis using PRK2 mouse mAb against PC-12 (1), Cos7 (2), K562 (3), Jurkat (4), Hela (5), A431 (6), C6 (7), NIH/3T3 (8) and HEK293 (9) cell lysate.
Figure 3: Immunohistochemical analysis of paraffin-embedded prostate tissues using PRK2 mouse mAb with DAB staining.
Figure 4: Immunohistochemical analysis of paraffin-embedded lung cancer tissues using PRK2 mouse mAb with DAB staining.
Figure 5: Flow cytometric analysis of NIH/3T3 cells using PRK2 mouse mAb (blue) and negative control (red).
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Protein-kinase-C-related kinases (PRKs) are part of the lipid-regulated protein kinases (PKC) which also include liver PAK & PKN. Human PRK1 and PRK2 share structurally similar catalytic domains, but less similar N-terminal regulatory regions suggesting different regulatory domain functions. PRK1 and PRK2, as well as a third member of this family, PRK3, show distinct patterns of expression in adult tissues. Additionally, the serine-threonine kinase PRK2 can be specifically cleaved by caspase-3 (and/or caspase-3-like subfamily members) during apoptosis.
|References (references)||1. Cell. 2009 Jul 23;138(2):389-403. |
2. Ann Intern Med. 2009 Apr 21;150(8):541-50.