PRDM1 Primary Antibody

Item Information
Catalog # Size/Concentration Price
30874 100μl $341.00
Specification
AliasesBLIMP1; PRDI-BF1
Product OrderP
Clone#2F1B6
Entrez GeneID639
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG2b
ImmunogenPurified recombinant fragment of human PRDM1 (AA: 690-825) expressed in E. Coli.
MW91.7kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C.  Learn More
Species ReactivityHuman
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
Sequence
690-825
Catalog
30874
$341.00
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Images

Elisa

Cell Culture Products
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

Western Blot

Cell Culture Products
Figure 2:Western blot analysis using PRDM1 mAb against human PRDM1 (AA: 690-825) recombinant protein. (Expected MW is 40.3 kDa)

Western Blot

Cell Culture Products
Figure 3:Western blot analysis using PRDM1 mAb against HEK293 (1) and PRDM1 (AA: 690-825)-hIgGFc transfected HEK293 (2) cell lysate.

Immunofluorescence analysis

Cell Culture Products
Figure 4:Immunofluorescence analysis of HeLa cells using PRDM1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric

Cell Culture Products
Figure 5:Flow cytometric analysis of Raji cells using PRDM1 mouse mAb (green) and negative control (red).
Product Overview
Description This gene encodes a protein that acts as a repressor of beta-interferon gene expression. The protein binds specifically to the PRDI (positive regulatory domain I element) of the beta-IFN gene promoter. Transcription of this gene increases upon virus induction. Two alternatively spliced transcript variants that encode different isoforms have been reported.
References (references)
References (references) 1.Proc Natl Acad Sci U S A. 2011 Dec 13;108(50):20119-24.
2.Mol Cancer Res. 2010 Jun;8(6):907-18.

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