PPY Primary Antibody
Item Information
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Size
Price
Description
This gene belongs to the NPY family and it encodes a protein that is synthesized as a 95 aa polypeptide precursor in the pancreatic islets of Langerhans. It is cleaved into two peptide products; the active hormone of 36 aa and an icosapeptide of unknown function. The hormone acts as a regulator of pancreatic and gastrointestinal functions and may be important in the regulation of food intake. Plasma level of this hormone has been shown to be reduced in conditions associated with increased food intake and elevated in anorexia nervosa. In addition, infusion of this hormone in obese rodents has shown to decrease weight gain.
Product Overview
Entrez GenelD
5539
Aliases
PNP
Clone#
2G5D12
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human PPY (AA: 1-95) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Am J Clin Nutr. 2011 Oct;94(4):967-72.
2.Pharm Res. 2012 Jun;29(6):1698-711.
2.Pharm Res. 2012 Jun;29(6):1698-711.
Product Image
Western Blot
Figure 1: Western blot analysis using PPY mAb against human PPY recombinant protein. (Expected MW is 35.9 kDa)
Flow cytometric
Figure 2: Flow cytometric analysis of HepG2 cells using PPY mouse mAb (green) and negative control (purple).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.