PPP1CA Primary Antibody
Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Studies in both human and mice suggest that PP1 is an important regulator of cardiac function. Mouse studies also suggest that PP1 functions as a suppressor of learning and memory. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Product Overview
Entrez GenelD
5499
Aliases
PP-1A; PPP1A; PP1alpha
Clone#
5E9
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human PPP1CA expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
ELISA
1/10000
References
1. J Biol Chem. 2009 Sep 18;284(38):25576-84.
2. Mol Cell Biol. 2009 Jun;29(12):3355-66.
2. Mol Cell Biol. 2009 Jun;29(12):3355-66.
Product Image
Western Blot
Figure 1: Western blot analysis using PPP1CA mAb against human PPP1CA (AA: 174-330) recombinant protein. (Expected MW is 43.4 kDa)
Western Blot
Figure 2: Western blot analysis using PPP1CA mouse mAb against Hela (1), HepG2 (2), MCF-7 (3), Jurkat (4) and A549 (5) cell lysate.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.