PPARA Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesPPAR; NR1C1; hPPAR; PPARalpha
ProductOrderP
Clone#5D10C3
Entrez GenelD5465
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human PPARA (AA: 1-120) expressed in E. Coli.
MW52.2kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman, Monkey
Application
ELISA1/10000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
1-120
Catalog#: 30858
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Images
Elisa
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western Blot
Figure 2:Western blot analysis using PPARA mAb against human PPARA (AA: 1-120) recombinant protein. (Expected MW is 38.4 kDa)
Figure 2:Western blot analysis using PPARA mAb against human PPARA (AA: 1-120) recombinant protein. (Expected MW is 38.4 kDa)
Western Blot
Figure 3:Western blot analysis using PPARA mAb against HEK293 (1) and PPARA (AA: 1-120)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using PPARA mAb against HEK293 (1) and PPARA (AA: 1-120)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using PPARA mouse mAb against HepG2 (1), HEK293 (2), COS7 (7), HepG2 (4), and Jurkat (5) cell lysate.
Figure 4:Western blot analysis using PPARA mouse mAb against HepG2 (1), HEK293 (2), COS7 (7), HepG2 (4), and Jurkat (5) cell lysate.
Flow cytometric
Figure 5:Flow cytometric analysis of Jurkat cells using PPARA mouse mAb (green) and negative control (red).
Figure 5:Flow cytometric analysis of Jurkat cells using PPARA mouse mAb (green) and negative control (red).
Product Overview
DescriptionPeroxisome proliferators include hypolipidemic drugs, herbicides, leukotriene antagonists, and plasticizers; this term arises because they induce an increase in the size and number of peroxisomes. Peroxisomes are subcellular organelles found in plants and animals that contain enzymes for respiration and for cholesterol and lipid metabolism. The action of peroxisome proliferators is thought to be mediated via specific receptors, called PPARs, which belong to the steroid hormone receptor superfamily. PPARs affect the expression of target genes involved in cell proliferation, cell differentiation and in immune and inflammation responses. Three closely related subtypes (alpha, beta/delta, and gamma) have been identified. This gene encodes the subtype PPAR-alpha, which is a nuclear transcription factor. Multiple alternatively spliced transcript variants have been described for this gene, although the full-length nature of only two has been determined.
References (references)
References (references)1.Blood. 2013 Aug 8;122(6):969-80.
2.Leukemia. 2013 Apr;27(5):1090-9.