PPARA Primary Antibody

Item Information
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Price
Description
Peroxisome proliferators include hypolipidemic drugs, herbicides, leukotriene antagonists, and plasticizers; this term arises because they induce an increase in the size and number of peroxisomes. Peroxisomes are subcellular organelles found in plants and animals that contain enzymes for respiration and for cholesterol and lipid metabolism. The action of peroxisome proliferators is thought to be mediated via specific receptors, called PPARs, which belong to the steroid hormone receptor superfamily. PPARs affect the expression of target genes involved in cell proliferation, cell differentiation and in immune and inflammation responses. Three closely related subtypes (alpha, beta/delta, and gamma) have been identified. This gene encodes the subtype PPAR-alpha, which is a nuclear transcription factor. Multiple alternatively spliced transcript variants have been described for this gene, although the full-length nature of only two has been determined.
Product Overview
Entrez GenelD
5465
Aliases
PPAR; NR1C1; hPPAR; PPARalpha
Clone#
5D10E10
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human PPARA (AA: 1-120) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Blood. 2013 Aug 8;122(6):969-80.
2.Leukemia. 2013 Apr;27(5):1090-9.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western Blot
Figure 2:Western blot analysis using PPARA mAb against human PPARA (AA: 1-120) recombinant protein. (Expected MW is 38.4 kDa)
Figure 2:Western blot analysis using PPARA mAb against human PPARA (AA: 1-120) recombinant protein. (Expected MW is 38.4 kDa)
Western Blot
Figure 3:Western blot analysis using PPARA mAb against HEK293 (1) and PPARA (AA: 1-120)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using PPARA mAb against HEK293 (1) and PPARA (AA: 1-120)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of Jurkat cells using PPARA mouse mAb (green) and negative control (red).
Figure 4:Flow cytometric analysis of Jurkat cells using PPARA mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.