PPARA Primary Antibody
|Aliases||PPAR; NR1C1; hPPAR; PPARalpha|
|Formulation||Purified antibody in PBS with 0.05% sodium azide|
|Immunogen||Purified recombinant fragment of human PPARA (AA: 1-120) expressed in E. Coli.|
|Shipping Information||This product will ship in a box containing blue ice at a temperature of 4°C. Learn More|
|FCM (Flow Cytometry)||1/200 - 1/400|
|WB (Western Blot)||1/500 - 1/2000|
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Figure 2:Western blot analysis using PPARA mAb against human PPARA (AA: 1-120) recombinant protein. (Expected MW is 38.4 kDa)
Figure 3:Western blot analysis using PPARA mAb against HEK293 (1) and PPARA (AA: 1-120)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Flow cytometric analysis of Jurkat cells using PPARA mouse mAb (green) and negative control (red).
|Description||Peroxisome proliferators include hypolipidemic drugs, herbicides, leukotriene antagonists, and plasticizers; this term arises because they induce an increase in the size and number of peroxisomes. Peroxisomes are subcellular organelles found in plants and animals that contain enzymes for respiration and for cholesterol and lipid metabolism. The action of peroxisome proliferators is thought to be mediated via specific receptors, called PPARs, which belong to the steroid hormone receptor superfamily. PPARs affect the expression of target genes involved in cell proliferation, cell differentiation and in immune and inflammation responses. Three closely related subtypes (alpha, beta/delta, and gamma) have been identified. This gene encodes the subtype PPAR-alpha, which is a nuclear transcription factor. Multiple alternatively spliced transcript variants have been described for this gene, although the full-length nature of only two has been determined. |
|References (references)||1.Blood. 2013 Aug 8;122(6):969-80. |
2.Leukemia. 2013 Apr;27(5):1090-9.