PMS2 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesPMSL2; HNPCC4; PMS2CL
ProductOrderP
Clone#1E9D11
Entrez GenelD5395
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human PMS2 (AA: 748-851) expressed in E. Coli.
MW95.8kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
748-851
Catalog#: 30863
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Images
Elisa
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western Blot
Figure 2:Western blot analysis using PMS2 mAb against human PMS2 (AA: 748-851) recombinant protein. (Expected MW is 37.7 kDa)
Figure 2:Western blot analysis using PMS2 mAb against human PMS2 (AA: 748-851) recombinant protein. (Expected MW is 37.7 kDa)
Western Blot
Figure 3:Western blot analysis using PMS2 mAb against HEK293 (1) and PMS2 (AA: 748-851)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using PMS2 mAb against HEK293 (1) and PMS2 (AA: 748-851)-hIgGFc transfected HEK293 (2) cell lysate.
Immunofluorescence analysis
Figure 4:Immunofluorescence analysis of HeLa cells using PMS2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 4:Immunofluorescence analysis of HeLa cells using PMS2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Immunofluorescence analysis
Figure 5:Immunofluorescence analysis of MCF-7 cells using PMS2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 5:Immunofluorescence analysis of MCF-7 cells using PMS2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 6:Flow cytometric analysis of HeLa cells using PMS2 mouse mAb (green) and negative control (red).
Figure 6:Flow cytometric analysis of HeLa cells using PMS2 mouse mAb (green) and negative control (red).
Product Overview
DescriptionThis gene is one of the PMS2 gene family members found in clusters on chromosome 7. The product of this gene is involved in DNA mismatch repair. It forms a heterodimer with MLH1 and this complex interacts with other complexes bound to mismatched bases. Mutations in this gene are associated with hereditary nonpolyposis colorectal cancer, Turcot syndrome, and are a cause of supratentorial primitive neuroectodermal tumors. Alternatively spliced transcript variants have been observed for this gene.
References (references)
References (references)1.J Med Genet. 2013 Aug;50(8):552-63.
2.Hum Mutat. 2010 May;31(5):552-60.