Mouse Monoclonal Antibody to PMEL
This gene encodes a melanocyte-specific type I transmembrane glycoprotein. The encoded protein is enriched in melanosomes, which are the melanin-producing organelles in melanocytes, and plays an essential role in the structural organization of premelanosomes. This protein is involved in generating internal matrix fibers that define the transition from Stage I to Stage II melanosomes. This protein undergoes a complex pattern of prosttranslational processing and modification that is essential to the proper functioning of the protein. A secreted form of this protein that is released by proteolytic ectodomain shedding may be used as a melanoma-specific serum marker. Alternate splicing results in multiple transcript variants.
![Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/811ee17ecc94d10095adece55eed61f33934f9fcc9379651ecbcf8c4e176bb79.jpg?v=1666705961)
![Figure 2:Western blot analysis using PMEL mAb against human PMEL (AA: 25-192) recombinant protein. (Expected MW is 44.7 kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/5971608396c90fc94ac04b32777195862831e20baba910f0c6114df3c0b87d6e.jpg?v=1666705961)
![Figure 3:Western blot analysis using PMEL mAb against HEK293-6e (1) and PMEL (AA: 25-192)-hIgGFc transfected HEK293-6e (2) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/89a87a279646f681f7b4fe16bcc380ba9ac03896eb7854aa761a0d9d4ecee5cc.jpg?v=1666705961)
![Figure 4:Flow cytometric analysis of B16 cells using PMEL mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/4dfba8ed9297a3fc4d751e70199ed280bdee13cb9c334a9ac278176639105c2b.jpg?v=1666705961)