PLIN3

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Description

Mannose 6-phophate receptors (MPRs) deliver lysosomal hydrolase from the Golgi to endosomes and then return to the Golgi complex. The protein encoded by this gene interacts with the cytoplasmic domains of both cation-independent and cation-dependent MPRs, and is required for endosome-to-Golgi transport. This protein also binds directly to the GTPase RAB9 (RAB9A), a member of the RAS oncogene family. The interaction with RAB9 has been shown to increase the affinity of this protein for its cargo. Multiple transcript variants encoding different isoforms have been found for this gene.

Product Overview
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
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Western Blot
Figure 1:Western blot analysis using PLIN3 mouse mAb against THP-1 (1), HepG2 (2), K562 (3) and Hela (4) cell lysate.
Figure 1:Western blot analysis using PLIN3 mouse mAb against THP-1 (1), HepG2 (2), K562 (3) and Hela (4) cell lysate.
Immunohistochemical analysis
Figure 2:Immunofluorescence analysis of Hela cells using PLIN3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 2:Immunofluorescence analysis of Hela cells using PLIN3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Immunofluorescence analysis
Figure 3:Flow cytometric analysis of Hela cells using PLIN3 mouse mAb (green) and negative control (red).
Figure 3:Flow cytometric analysis of Hela cells using PLIN3 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.