PLIN2 Primary Antibody
The protein encoded by this gene belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases. Alternatively spliced transcript variants have been found for this gene.
Purified antibody in PBS with 0.05% sodium azide
4°C; -20°C for long term storage
Figure 1: Western blot analysis using PLIN2 mAb against human PLIN2 (AA: 286-437) recombinant protein. (Expected MW is 42.6 kDa)
Figure 2: Western blot analysis using PLIN2 mAb against HEK293 (1) and PLIN2 (AA: 286-437)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3: Western blot analysis using PLIN2 mouse mAb against HepG2 cell lysate.
Figure 4: Immunofluorescence analysis of HepG2 cells using PLIN2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Secondary antibody from Fisher (Cat#: 35503)
Figure 5: Flow cytometric analysis of HepG2 cells using PLIN2 mouse mAb (green) and negative control (red).
Figure 6: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using PLIN2 mouse mAb with DAB staining.
Figure 7: Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using PLIN2 mouse mAb with DAB staining.
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.