PLIN2 Primary Antibody
Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases. Alternatively spliced transcript variants have been found for this gene.
Product Overview
Entrez GenelD
123
Aliases
ADFP; ADRP
Clone#
2C5H8
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human PLIN2 (AA: 286-437) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1. Am J Physiol Endocrinol Metab. 2012 Nov 1;303(9):E1158-65.
2. Exp Physiol. 2012 Aug;97(8):970-80.
2. Exp Physiol. 2012 Aug;97(8):970-80.
Product Image
Western Blot
Figure 1: Western blot analysis using PLIN2 mAb against human PLIN2 (AA: 286-437) recombinant protein. (Expected MW is 42.6 kDa)
Western Blot
Figure 2: Western blot analysis using PLIN2 mAb against HEK293 (1) and PLIN2 (AA: 286-437)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 3: Western blot analysis using PLIN2 mouse mAb against HepG2 cell lysate.
Immunofluorescence analysis
Figure 4: Immunofluorescence analysis of HepG2 cells using PLIN2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 5: Flow cytometric analysis of HepG2 cells using PLIN2 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 6: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using PLIN2 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 7: Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using PLIN2 mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.