PLCG2 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesFCAS3; APLAID; PLC-IV; PLC-gamma-2
ProductOrderP
Clone#3A8B6
Entrez GenelD5336
FormulationPurified antibody in PBS with 0.05% sodium azide.
HostMouse
IsotypeIgG2b
ImmunogenSynthesized peptide of human PLCG2 (AA: phospho-Tyrosine 753 of human Phospholipase Cg2(cERDINSLpYDVSRMYV)).
MW148kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
phospho-Tyrosine 753 of human Phospholipase Cg2(cERDINSLpYDVSRMYV)
Catalog#: 30725
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Images
Western Blot
Figure 1: Western blot analysis using PLCG2 mouse mAb against A431 cell lysate.
Figure 1: Western blot analysis using PLCG2 mouse mAb against A431 cell lysate.
Immunofluorescence analysis
Figure 2:Immunofluorescence analysis of Hela cells using PLCG2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 2:Immunofluorescence analysis of Hela cells using PLCG2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 3: Flow cytometric analysis of Hela cells using PLCG2 mouse mAb (green) and negative control (red).
Figure 3: Flow cytometric analysis of Hela cells using PLCG2 mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Product Overview
Description

The protein encoded by this gene is a transmembrane signaling enzyme that catalyzes the conversion of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate to 1D-myo-inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) using calcium as a cofactor. IP3 and DAG are second messenger molecules important for transmitting signals from growth factor receptors and immune system receptors across the cell membrane. Mutations in this gene have been found in autoinflammation, antibody deficiency, and immune dysregulation syndrome and familial cold autoinflammatory syndrome 3.

References (references)
References (references)1. Mol Cell Biol. 2011 Mar;31(6):1240-51.
2. Am J Hum Genet. 2012 Oct 5;91(4):713-20.
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