PLAU

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesATF; QPD; UPA; URK; u-PA; BDPLT5
Clone#6B6A3
Entrez GenelD5328
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeMouse IgG1
ImmunogenPurified recombinant fragment of human PLAU (AA: 107-379) expressed in E. Coli.
MW48.5KDa
Species ReactivityHuman
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
107-379
Catalog#: 32275
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Images
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using PLAU mAb against human PLAU (AA: 107-379) recombinant protein. (Expected MW is 34 kDa)
Figure 2:Western blot analysis using PLAU mAb against human PLAU (AA: 107-379) recombinant protein. (Expected MW is 34 kDa)
Western Blot
Figure 4:Western blot analysis using PLAU mouse mAb against PC-3 (1),MCF-7 (2), LNCap (3),DU145 (4),HCT116 (5),A549 (6),SK-OV-3 (7) and HEK293 (8) cell lysate.
Figure 4:Western blot analysis using PLAU mouse mAb against PC-3 (1),MCF-7 (2), LNCap (3),DU145 (4),HCT116 (5),A549 (6),SK-OV-3 (7) and HEK293 (8) cell lysate.
Immunohistochemical analysis
Figure 5:Immunofluorescence analysis of Hela cells using PLAU mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 5:Immunofluorescence analysis of Hela cells using PLAU mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Immunofluorescence analysis
Figure 6:Flow cytometric analysis of Hela cells using PLAU mouse mAb (green) and negative control (red).
Figure 6:Flow cytometric analysis of Hela cells using PLAU mouse mAb (green) and negative control (red).
Immunofluorescence analysis
Figure 7:Flow cytometric analysis of HepG2 cells using PLAU mouse mAb (green) and negative control (red).
Figure 7:Flow cytometric analysis of HepG2 cells using PLAU mouse mAb (green) and negative control (red).
Product Overview
Description

This gene encodes a secreted serine protease that converts plasminogen to plasmin. The encoded preproprotein is proteolytically processed to generate A and B polypeptide chains. These chains associate via a single disulfide bond to form the catalytically inactive high molecular weight urokinase-type plasminogen activator (HMW-uPA). HMW-uPA can be further processed into the catalytically active low molecular weight urokinase-type plasminogen activator (LMW-uPA). This low molecular weight form does not bind to the urokinase-type plasminogen activator receptor. Mutations in this gene may be associated with Quebec platelet disorder and late-onset Alzheimer's disease. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed.

References (references)
References (references)1.Biochim Biophys Acta Proteins Proteom.2021 Feb;1869(2):140562.
2.Molecules.2021 Mar 24;26(7):1816.