PLA2G12A Primary Antibody
Item Information
Catalog #
Size
Price
Description
Secreted phospholipase A2 (sPLA2) enzymes liberate arachidonic acid from phospholipids for production of eicosanoids and exert a variety of physiologic and pathologic effects. Group XII sPLA2s, such as PLA2G12A, have relatively low specific activity and are structurally and functionally distinct from other sPLA2s
Product Overview
Entrez GenelD
81579
Aliases
GXII; ROSSY; PLA2G12
Clone#
3H2C11
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human PLA2G12A (AA: 21-189) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide.
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1. J Biol Chem. 2003 Mar 21;278(12):10657-67.
2. Biochemistry. 2003 Oct 7;42(39):11494-503.
2. Biochemistry. 2003 Oct 7;42(39):11494-503.
Product Image
Western Blot
Figure 1: Western blot analysis using PLA2G12A mAb against human PLA2G12A (AA: 21-189) recombinant protein. (Expected MW is 44.5 kDa)
Western Blot
Figure 2: Western blot analysis using PLA2G12A mAb against HEK293 (1) and PLA2G12A (AA: 21-189)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 3: Flow cytometric analysis of Hela cells using PLA2G12A mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 4: Immunohistochemical analysis of paraffin-embedded liver cancer tissues using PLA2G12A mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.