PKN1 Primary Antibody

Item Information

Catalog #

Size

Price

Description

The protein encoded by this gene belongs to the protein kinase C superfamily. This kinase is activated by Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can be activated by phospholipids and by limited proteolysis. The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase, which may mediate insulin signals to the actin cytoskeleton. The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Product Overview

Entrez GenelD

5585

Aliases

DBK; PKN; PAK1; PRK1; PAK-1; PRKCL1; PKN-ALPHA

Clone#

4H10B1

Host / Isotype

Mouse / IgG2b

Species Reactivity

Human

Immunogen

Purified recombinant fragment of human PKN1 (AA: 442-620) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide.

Storage

4°C; -20°C for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

ICC (Immunocytochemistry)

1/200 - 1/1000

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1. J Biol Chem. 2013 Nov 29;288(48):34658-70.
2. Hum Pathol. 2009 Oct;40(10):1434-40.

Product Image

Western Blot

Figure 1: Western blot analysis using PKN1 mAb against human PKN1 (AA: 442-620) recombinant protein. (Expected MW is 45.7 kDa)

Western Blot

Figure 2: Western blot analysis using PKN1 mAb against HEK293 (1) and PKN1 (AA: 442-620)-hIgGFc transfected HEK293 (2) cell lysate.

Immunofluorescence analysis

Figure 3:Immunofluorescence analysis of Hela cells using PKN1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric