PKN1 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesDBK; PKN; PAK1; PRK1; PAK-1; PRKCL1; PKN-ALPHA
ProductOrderP
Clone#4H10B1
Entrez GenelD5585
FormulationPurified antibody in PBS with 0.05% sodium azide.
HostMouse
IsotypeIgG2b
ImmunogenPurified recombinant fragment of human PKN1 (AA: 442-620) expressed in E. Coli.
MW104kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
442-620
Catalog#: 30717
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Images
Western Blot
Figure 1: Western blot analysis using PKN1 mAb against human PKN1 (AA: 442-620) recombinant protein. (Expected MW is 45.7 kDa)
Figure 1: Western blot analysis using PKN1 mAb against human PKN1 (AA: 442-620) recombinant protein. (Expected MW is 45.7 kDa)
Western Blot
Figure 2: Western blot analysis using PKN1 mAb against HEK293 (1) and PKN1 (AA: 442-620)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 2: Western blot analysis using PKN1 mAb against HEK293 (1) and PKN1 (AA: 442-620)-hIgGFc transfected HEK293 (2) cell lysate.
Immunofluorescence analysis
Figure 3:Immunofluorescence analysis of Hela cells using PKN1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 3:Immunofluorescence analysis of Hela cells using PKN1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 4: Flow cytometric analysis of Hela cells using PKN1 mouse mAb (green) and negative control (red).
Figure 4: Flow cytometric analysis of Hela cells using PKN1 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 5: Immunohistochemical analysis of paraffin-embedded colon cancer tissues using PKN1 mouse mAb with DAB staining.
Figure 5: Immunohistochemical analysis of paraffin-embedded colon cancer tissues using PKN1 mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Product Overview
Description

The protein encoded by this gene belongs to the protein kinase C superfamily. This kinase is activated by Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can be activated by phospholipids and by limited proteolysis. The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase, which may mediate insulin signals to the actin cytoskeleton. The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

References (references)
References (references)1. J Biol Chem. 2013 Nov 29;288(48):34658-70.
2. Hum Pathol. 2009 Oct;40(10):1434-40.