PINCH Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesPINCH; PINCH1; PINCH-1; LIMS1
ProductOrderP
Clone#5G7
Entrez GenelD3987
FormulationAscitic fluid containing 0.03% sodium azide.
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human PINCH expressed in E. Coli.
MW37kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
87-249
Catalog#: 30241
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510-740-3625
info@promab.com
Images
Western Blot
Figure 1: Western blot analysis using PINCH mAb against human PINCH (AA: 87-249) recombinant protein. (Expected MW is 44.2 kDa)
Figure 1: Western blot analysis using PINCH mAb against human PINCH (AA: 87-249) recombinant protein. (Expected MW is 44.2 kDa)
Western Blot
Figure 2: Western blot analysis using PINCH mouse mAb against A549 (1), Jurkat (2), and Hela (3) cell lysate.
Figure 2: Western blot analysis using PINCH mouse mAb against A549 (1), Jurkat (2), and Hela (3) cell lysate.
Immunofluorescence analysis
Figure 3: Immunofluorescence analysis of HepG2 cells using PINCH mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Figure 3: Immunofluorescence analysis of HepG2 cells using PINCH mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric
Figure 4: Flow cytometric analysis of Hela cells using PINCH mouse mAb (blue) and negative control (red).
Figure 4: Flow cytometric analysis of Hela cells using PINCH mouse mAb (blue) and negative control (red).
Elisa
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Product Overview
Description

The protein encoded by this gene is an adaptor protein which contains five LIM domains, or double zinc fingers. The protein is likely involved in integrin signaling through its LIM domain-mediated interaction with integrin-linked kinase, found in focal adhesion plaques. It is also thought to act as a bridge linking integrin-linked kinase to NCK adaptor protein 2, which is involved in growth factor receptor kinase signaling pathways. Its localization to the periphery of spreading cells also suggests that this protein may play a role in integrin-mediated cell adhesion or spreading. Several transcript variants encoding different isoforms have been found for this gene.

References (references)
References (references)1. J Biol Chem. 2009 Feb 27;284(9):5836-44.
2. Proc Natl Acad Sci U S A. 2008 Dec 30;105(52):20677-82.
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