PIK3CA Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesPI3K; p110-alpha
ProductOrderP
Clone#4F3
Entrez GenelD5290
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human PIK3CA expressed in E. Coli.
MW124kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
881-1068
Catalog#: 30334
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Images
Western Blot
Figure 1: Western blot analysis using PIK3CA mAb against human PIK3CA (AA: 881-1068) recombinant protein. (Expected MW is 47.4 kDa)
Figure 1: Western blot analysis using PIK3CA mAb against human PIK3CA (AA: 881-1068) recombinant protein. (Expected MW is 47.4 kDa)
Western Blot
Figure 2: Western blot analysis using PIK3CA mAb against HEK293 (1) and PIK3CA (AA: 881-1068)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 2: Western blot analysis using PIK3CA mAb against HEK293 (1) and PIK3CA (AA: 881-1068)-hIgGFc transfected HEK293 (2) cell lysate.
Immunofluorescence analysis
Figure 3: Immunofluorescence analysis of HeLa cells using PIK3CA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Figure 3: Immunofluorescence analysis of HeLa cells using PIK3CA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Flow cytometric
Figure 4: Flow cytometric analysis of Jurkat cells using PIK3CA mouse mAb (green) and negative control (red).
Figure 4: Flow cytometric analysis of Jurkat cells using PIK3CA mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Product Overview
Description

Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers.

References (references)
References (references)Cancer Res. 2009 Dec 1;69(23):8868-76.
Diagn Mol Pathol. 2009 Dec;18(4):200-5.