PGRMC1 Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene encodes a putative membrane-associated progesterone steroid receptor. The protein is expressed predominantly in the liver and kidney.
Product Overview
Entrez GenelD
10857
Aliases
MPR; HPR6.6
Clone#
6F9A1
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human PGRMC1 (AA:1-195) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Cancer Lett. 2015 Jan 28;356(2 Pt B):434-42.
2.Nan Fang Yi Ke Da Xue Xue Bao. 2012 May;32(5):635-8.
2.Nan Fang Yi Ke Da Xue Xue Bao. 2012 May;32(5):635-8.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using PGRMC1 mAb against human PGRMC1 (AA: 1-195) recombinant protein. (Expected MW is 47.6 kDa)
Western Blot
Figure 3:Western blot analysis using PGRMC1 mAb against HEK293 (1) and PGRMC1 (AA: 1-195)-hIgGFc transfected HEK293 (2) cell lysate.
Immunofluorescence analysis
Figure 4:Immunofluorescence analysis of MCF-7 cells using PGRMC1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Immunofluorescence analysis
Figure 5:Immunofluorescence analysis of SK-OV-3 cells using PGRMC1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 6:Flow cytometric analysis of A549 cells using PGRMC1 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded lung cancer tissues using PGRMC1 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 8:Immunohistochemical analysis of paraffin-embedded breast cancer tissues using PGRMC1 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.