PEG10 Primary Antibody

Item Information
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Price
Description

PEG10: paternally expressed 10. This gene includes two overlapping reading frames of the same transcript encoding distinct isoforms. The shorter isoform has a CCHC-type zinc finger motif containing a sequence characteristic of gag proteins of most retroviruses and some retrotransposons, and it functions in part by interacting with members of the TGF-beta receptor family. The longer isoform has the active-site DSG consensus sequence of the protease domain of pol proteins. The longer isoform is the result of -1 translational frameshifting that is also seen in some retroviruses. Expression of these two isoforms only comes from the paternal allele due to imprinting. Increased gene expression (as observed by an increase in mRNA levels) is associated with hepatocellular carcinomas.

Product Overview
Entrez GenelD
23089
Aliases
Edr; HB-1; Mar2; MEF3L; Mart2; RGAG3; KIAA1051
Clone#
1B1C4
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of PEG10 (aa1-120) expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
ELISA
1/10000
References
1. Oncogene. 2007 Aug 23;26(39):5741-51.
2. FEBS Lett. 2008 Aug 6;582(18):2793-8.
Product Image
Western Blot
Figure 1: Western blot analysis using PEG10 mouse mAb against truncated Trx-PEG10 recombinant protein (1),truncated GST-PEG10 (aa1-120) recombinant protein (2) and full-length PEG10 (aa1-325)-hIgGFc transfected CHO-K1 cell lysate (3).
Figure 1: Western blot analysis using PEG10 mouse mAb against truncated Trx-PEG10 recombinant protein (1),truncated GST-PEG10 (aa1-120) recombinant protein (2) and full-length PEG10 (aa1-325)-hIgGFc transfected CHO-K1 cell lysate (3).
Immunohistochemical analysis
Figure 2: Immunohistochemical analysis of paraffin-embedded human Placenta tissues using PEG10 mouse mAb
Figure 2: Immunohistochemical analysis of paraffin-embedded human Placenta tissues using PEG10 mouse mAb
Immunofluorescence analysis
Figure 3: Confocal Immunofluorescence analysis of methanol-fixed HepG2 cells using PEG10 mouse mAb (green), showing cytoplasmic localization. Blue: DRAQ5 fluorescent DNA dye.
Figure 3: Confocal Immunofluorescence analysis of methanol-fixed HepG2 cells using PEG10 mouse mAb (green), showing cytoplasmic localization. Blue: DRAQ5 fluorescent DNA dye.
For Research Use Only. Not for use in diagnostic procedures.