PDK1 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesPDK1
ProductOrderP
Clone#4A11
Entrez GenelD5163
FormulationAscitic fluid containing 0.03% sodium azide.
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human PDK1 expressed in E. Coli.
MW44kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman, Monkey, Rat
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
308-436
Catalog#: 30154
Inquire
Contact us to order
1-866-339-0871
510-740-3625
info@promab.com
Images
Western Blot
Figure 1: Western blot analysis using PDK1 mouse mAb against NIH/3T3 (1), Hela (2), Jurkat (3), HepG2 (4), PC-12 (5), and Cos7 (6) cell lysate.
Figure 1: Western blot analysis using PDK1 mouse mAb against NIH/3T3 (1), Hela (2), Jurkat (3), HepG2 (4), PC-12 (5), and Cos7 (6) cell lysate.
Immunohistochemical analysis
Figure 2: Immunohistochemical analysis of paraffin-embedded breast cancer tissues (left) and brain tissues (right) using PDK1 mouse mAb with DAB staining.
Figure 2: Immunohistochemical analysis of paraffin-embedded breast cancer tissues (left) and brain tissues (right) using PDK1 mouse mAb with DAB staining.
Immunofluorescence analysis
Figure 3: Immunofluorescence analysis of HELA cells using PDK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Figure 3: Immunofluorescence analysis of HELA cells using PDK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric
Figure 4: Flow cytometric analysis of Lovo cells using PDK1 mouse mAb (green) and negative control (purple).
Figure 4: Flow cytometric analysis of Lovo cells using PDK1 mouse mAb (green) and negative control (purple).
Product Overview
Description

Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation. (provided by RefSeq). Tissue specificity: Expressed predominantly in the heart.

References (references)
References (references)1. Nat Cell Biol. 2008 Feb;10(2):127-37.
2. Blood. 2008 Apr 1;111(7):3723-34.
3. J Biol Chem. 2007 Apr 20;282(16):12272-89.
Please view FAQs here