PDHA1
The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
2.Pathol Res Pract. 2019 Mar;215(3):478-482.
![Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/32407ea.jpg?v=1669029722)
![Figure 2:Western blot analysis using PDHA1 mAb against human PDHA1 (AA: 241-390) recombinant protein. (Expected MW is 37.5 kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/32407rwb.jpg?v=1669029722)
![Figure 3:Western blot analysis using PDHA1 mAb against HEK293-6e (1) and PDHA1 (AA: 241-390)-hIgGFc transfected HEK293-6e (2) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/32407twb.jpg?v=1669029722)
![Figure 4:Western blot analysis using PDHA1 mouse mAb against HepG2 (1), HL-60 (2),SK-OV-3 (3),PC-3 (4) and PANC-1 (5) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/32407lwb.jpg?v=1669029722)
![Figure 5:Flow cytometric analysis of Hela cells using PDHA1 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/32407fcm.jpg?v=1669029722)