PCNA Primary Antibody

Item Information

Catalog #

Size

Price

Description

The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.

Product Overview

Entrez GenelD

5111

Clone#

7H4F8

Host / Isotype

Mouse / IgG1

Species Reactivity

Human, Monkey

Immunogen

Purified recombinant fragment of human PCNA (AA: 53-196 ) expressed in E. Coli.

Formulation

Ascitic fluid containing 0.03% sodium azide.

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1.Cancer Res. 2012 Jul 1;72(13):3217-27.
2.PLoS One. 2012;7(1):e29416.

Product Image

Western Blot

Figure 1: Western blot analysis using PCNA mAb against human PCNA recombinant protein. (Expected MW is 41.2 kDa)

Western Blot

Figure 2: Western blot analysis using PCNA mAb against HEK293 (1) and PCNA (AA: 53-196)-hIgGFc transfected HEK293 (2) cell lysate.

Western Blot

Figure 3: Western blot analysis using PCNA mouse mAb against A431 (1), HEK293 (2), HeLa (3), HepG2 (4), Raji (5), MOLT4 (6), COS7 (7), and MCF-7 (8) cell lysate.

Flow cytometric