PCK2 Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene encodes a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of guanosine triphosphate (GTP). A cytosolic form of this protein is encoded by a different gene and is the key enzyme of gluconeogenesis in the liver. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2014]
Product Overview
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using PCK2 mAb against human PCK2 (AA: 44-175) recombinant protein. (Expected MW is 40.5 kDa)
Western Blot
Figure 3:Western blot analysis using PCK2 mAb against HEK293 (1) and PCK2 (AA: 44-175)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using PCK2 mouse mAb against Jurkat (1), C2C12 (2), Hela (3), HepG2 (4), COS7 (5), and HL-60 (6) cell lysate.
Immunofluorescence analysis
Figure 5:Immunofluorescence analysis of Hela cells using PCK2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 6:Flow cytometric analysis of Hela cells using PCK2 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using PCK2 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.