PAX8 Primary Antibody

Item Information

Catalog #

Size

Price

Description

This gene encodes a member of the paired box (PAX) family of transcription factors. Members of this gene family typically encode proteins that contain a paired box domain, an octapeptide, and a paired-type homeodomain. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas and atypical follicular thyroid adenomas. Alternatively spliced transcript variants encoding different isoforms have been described.

Product Overview

Entrez GenelD

7849

Clone#

1A1D3

Host / Isotype

Mouse / Mouse IgG1

Immunogen

Purified recombinant fragment of human PAX8 (AA: 60-261) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

4°C; -20°C for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200-1/1000

FCM (Flow Cytometry)

1/200-1/400

ELISA

1/10000

References

1.BMC Mol Cell Biol. 2019 Dec 27;20(1):61. 2.PLoS One. 2017 Mar 24;12(3):e0173700.

Product Image

ELISA

Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)

WESTERN BLOT

Figure 2: Western blot analysis using PAX8 mAb against human PAX8 (AA: 60-261) recombinant protein. (Expected MW is 25 kDa)

WESTERN BLOT

Figure 3: Western blot analysis using PAX8 mAb against HEK293-6e (1) and PAX8 (AA: 60-261)-hIgGFc transfected HEK293 (2) cell lysate.

WESTERN BLOT

Figure 4: Western blot analysis using PAX8 mouse mAb against HL-60 (1), HEK293 (2), Raji (3), Hela (4), Jurkat (5), A431 (6), A549 (7), and K562 (8) cell lysate.

FLOW CYTOMETRY