PAX6 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesAN; AN2; FVH1; MGDA; WAGR; D11S812E
ProductOrderP
Clone#1F6G2
Entrez GenelD5080
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human PAX6 (AA: 1-122) expressed in E. Coli.
MW46.7kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
1-122
Catalog#: 30845
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Images
Elisa
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western Blot
Figure 2:Western blot analysis using PAX6 mAb against human PAX6 (AA: 1-122) recombinant protein. (Expected MW is 39.2 kDa)
Figure 2:Western blot analysis using PAX6 mAb against human PAX6 (AA: 1-122) recombinant protein. (Expected MW is 39.2 kDa)
Western Blot
Figure 3:Western blot analysis using PAX6 mAb against HEK293 (1) and PAX6 (AA: 1-122)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using PAX6 mAb against HEK293 (1) and PAX6 (AA: 1-122)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of A549 cells using PAX6 mouse mAb (green) and negative control (red).
Figure 4:Flow cytometric analysis of A549 cells using PAX6 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 5:Immunohistochemical analysis of paraffin-embedded colon cancer tissues using PAX6 mouse mAb with DAB staining.
Figure 5:Immunohistochemical analysis of paraffin-embedded colon cancer tissues using PAX6 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using PAX6 mouse mAb with DAB staining.
Figure 6:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using PAX6 mouse mAb with DAB staining.
Product Overview
DescriptionThis gene encodes paired box gene 6, one of many human homologs of the Drosophila melanogaster gene prd. In addition to the hallmark feature of this gene family, a conserved paired box domain, the encoded protein also contains a homeo box domain. Both domains are known to bind DNA and function as regulators of gene transcription. This gene is expressed in the developing nervous system, and in developing eyes. Mutations in this gene are known to cause ocular disorders such as aniridia and Peter's anomaly. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
References (references)
References (references)1.PLoS One. 2014 Jan 15;9(1):e85738.
2.Curr Eye Res. 2012 Oct;37(10):879-83.