PAX6 Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene encodes paired box gene 6, one of many human homologs of the Drosophila melanogaster gene prd. In addition to the hallmark feature of this gene family, a conserved paired box domain, the encoded protein also contains a homeo box domain. Both domains are known to bind DNA and function as regulators of gene transcription. This gene is expressed in the developing nervous system, and in developing eyes. Mutations in this gene are known to cause ocular disorders such as aniridia and Peter's anomaly. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Product Overview
Entrez GenelD
5080
Aliases
AN; AN2; FVH1; MGDA; WAGR; D11S812E
Clone#
1F6G2
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human PAX6 (AA: 1-122) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.PLoS One. 2014 Jan 15;9(1):e85738.
2.Curr Eye Res. 2012 Oct;37(10):879-83.
2.Curr Eye Res. 2012 Oct;37(10):879-83.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western Blot
Figure 2:Western blot analysis using PAX6 mAb against human PAX6 (AA: 1-122) recombinant protein. (Expected MW is 39.2 kDa)
Western Blot
Figure 3:Western blot analysis using PAX6 mAb against HEK293 (1) and PAX6 (AA: 1-122)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of A549 cells using PAX6 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 5:Immunohistochemical analysis of paraffin-embedded colon cancer tissues using PAX6 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using PAX6 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.