PAX6 Primary Antibody
|Aliases||AN; AN2; FVH1; MGDA; WAGR; D11S812E|
|Formulation||Purified antibody in PBS with 0.05% sodium azide|
|Immunogen||Purified recombinant fragment of human PAX6 (AA: 1-122) expressed in E. Coli.|
|Shipping Information||This product will ship in a box containing blue ice at a temperature of 4°C. Learn More|
|IHC_P(Immunohistochemistry)||1/200 - 1/1000|
|FCM (Flow Cytometry)||1/200 - 1/400|
|WB (Western Blot)||1/500 - 1/2000|
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Figure 2:Western blot analysis using PAX6 mAb against human PAX6 (AA: 1-122) recombinant protein. (Expected MW is 39.2 kDa)
Figure 3:Western blot analysis using PAX6 mAb against HEK293 (1) and PAX6 (AA: 1-122)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Flow cytometric analysis of A549 cells using PAX6 mouse mAb (green) and negative control (red).
Figure 5:Immunohistochemical analysis of paraffin-embedded colon cancer tissues using PAX6 mouse mAb with DAB staining.
Figure 6:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using PAX6 mouse mAb with DAB staining.
|Description||This gene encodes paired box gene 6, one of many human homologs of the Drosophila melanogaster gene prd. In addition to the hallmark feature of this gene family, a conserved paired box domain, the encoded protein also contains a homeo box domain. Both domains are known to bind DNA and function as regulators of gene transcription. This gene is expressed in the developing nervous system, and in developing eyes. Mutations in this gene are known to cause ocular disorders such as aniridia and Peter's anomaly. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.|
|References (references)||1.PLoS One. 2014 Jan 15;9(1):e85738. |
2.Curr Eye Res. 2012 Oct;37(10):879-83.