p53 Primary Antibody

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Description

p53 responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Alterations of this gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome. Multiple p53 variants due to alternative promoters and multiple alternative splicing have been found. These variants encode distinct isoforms, which can regulate p53 transcriptional activity.

Product Overview
Entrez GenelD
7157
Aliases
LFS1, TRP53, TP53
Clone#
4A8
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human p53 expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ELISA
1/10000
References
1. Cancer Invest. 2009 Jan;27(1):96-104.
2. Anticancer Res. 2007 Nov-Dec;27(6B):4143-8.
3. Mol Cell. 2008 Feb 1;29(2):217-31.
Product Image
Western Blot
Figure 1: Western blot analysis using p53 mouse mAb against HEK293 cell lysate(1).
Figure 1: Western blot analysis using p53 mouse mAb against HEK293 cell lysate(1).
Immunohistochemical analysis
Figure 2: Immunohistochemical analysis of paraffin-embedded human esophageal cancer (left) and lung cancer (right), showing nuclear localization using p53 mouse mAb with DAB staining.
Figure 2: Immunohistochemical analysis of paraffin-embedded human esophageal cancer (left) and lung cancer (right), showing nuclear localization using p53 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.