p44/42 MAPK (Erk1/2) Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesERK; p38; p40; p41; ERK2; ERT1; MAPK2; PRKM1; PRKM2; P42MAPK; p41mapk; MAPK1
ProductOrderP
Clone#3F8
Entrez GenelD5594
FormulationAscitic fluid containing 0.03% sodium azide.
HostMouse
IsotypeIgG2b
ImmunogenPurified recombinant fragment of human MAPK expressed in E. Coli.
MW42kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman, Mouse
Application
ELISA1/10000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
160-360
Catalog#: 30014
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Images
Western Blot
Figure 1: Western blot analysis using p44/42 MAPK mouse mAb against Jurkat (1), Hela (2), A431 (3) and NIH/3T3 (4) cell lysate.
Figure 1: Western blot analysis using p44/42 MAPK mouse mAb against Jurkat (1), Hela (2), A431 (3) and NIH/3T3 (4) cell lysate.
Immunohistochemical analysis
Figure 2: Immunohistochemical analysis of paraffin-embedded human Liver tissues using anti-BHMT mouse mAb
Figure 2: Immunohistochemical analysis of paraffin-embedded human Liver tissues using anti-BHMT mouse mAb
Flow cytometric
Figure 3: Flow cytometric analysis of Jurkat cells using p44/42 MAPK mAb (green) and negative control (purple).
Figure 3: Flow cytometric analysis of Jurkat cells using p44/42 MAPK mAb (green) and negative control (purple).
Product Overview
Description

Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines and is an important target in the diagnosis and treatment of cancer. Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family as well as Mos and Tpl2/Cot. MEK1 and MEK2 are the primary MAPKKs in this pathway. MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK and the transcription factor Elk-1. p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs, along with MEK inhibitors such as U0126 and PD98059.

References (references)
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