P2RY4 Primary Antibody

Item Information

Catalog #

Size

Price

Description

The product of this gene belongs to the family of G-protein coupled receptors. This family has several receptor subtypes with different pharmacological selectivity, which overlaps in some cases, for various adenosine and uridine nucleotides. This receptor is responsive to uridine nucleotides, partially responsive to ATP, and not responsive to ADP.

Product Overview

Entrez GenelD

5030

Aliases

NRU; P2P; UNR; P2Y4

Clone#

3B4H6

Host / Isotype

Mouse / IgG1

Species Reactivity

Human

Immunogen

Purified recombinant fragment of human P2RY4 (AA: extra mix) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

4°C; -20°C for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

ICC (Immunocytochemistry)

1/100 - 1/500

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1.Arch Biochem Biophys. 2010 Feb 1;494(1):7-14.
2.Biochim Biophys Acta. 2009 Dec;1790(12):1651-9.

Product Image

Elisa

Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using P2RY4 mAb against human P2RY4 (AA: extra mix) recombinant protein. (Expected MW is 10.1 kDa)

Western Blot

Figure 3:Western blot analysis using P2RY4 mAb against HEK293 (1) and P2RY4 (AA: extra mix)-hIgGFc transfected HEK293 (2) cell lysate.

Immunofluorescence analysis

Figure 4:Immunofluorescence analysis of Hela cells using P2RY4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric