OTX2 Primary Antibody

Item Information

Catalog #

Size

Price

Description

This gene encodes a member of the bicoid sub-family of homeodomain-containing transcription factors. The encoded protein acts as a transcription factor and may play a role in brain and sensory organ development. A similar protein in mice is required for proper forebrain development.Tissue specificity: Expressed in brain.

Product Overview

Entrez GenelD

5015

Aliases

MCOPS5; MGC45000

Clone#

1H12C4B5

Host / Isotype

Mouse / IgG1

Species Reactivity

Human

Immunogen

Purified recombinant fragment of human OTX2 expressed in E. Coli.

Formulation

Ascitic fluid containing 0.03% sodium azide.

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

ICC (Immunocytochemistry)

1/200 - 1/1000

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1. Hum Mutat. 2008 Nov;29(11):E278-83.
2. Cancer Res. 2010 Jan 1;70(1):181-91.

Product Image

Western Blot

Figure 1: Western blot analysis using OTX2 mouse mAb against HepG2 (1), Jurkat (2), and NTERA-2 (3) cell lysate.

Western Blot

Figure 2: Western blot analysis using OTX2 mAb against human OTX2 (AA: 40-297) recombinant protein. (Expected MW is 65 kDa)

Immunohistochemical analysis

Figure 3: Immunohistochemical analysis of paraffin-embedded prostate tissues (left) and colon cancer tissues (right) using OTX2 mouse mAb with DAB staining.

Immunofluorescence analysis

Figure 4: Immunofluorescence analysis of HepG2 cells using OTX2 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Flow cytometric