NRCAM Primary Antibody

Item Information

Catalog #

Size

Price

Description

Cell adhesion molecules (CAMs) are members of the immunoglobulin superfamily. This gene encodes a neuronal cell adhesion molecule with multiple immunoglobulin-like C2-type domains and fibronectin type-III domains. This ankyrin-binding protein is involved in neuron-neuron adhesion and promotes directional signaling during axonal cone growth. This gene is also expressed in non-neural tissues and may play a general role in cell-cell communication via signaling from its intracellular domain to the actin cytoskeleton during directional cell migration. Allelic variants of this gene have been associated with autism and addiction vulnerability. Alternative splicing results in multiple transcript variants encoding different isoforms.

Product Overview

Entrez GenelD

4897

Aliases

NRCAM

Clone#

7D8C5

Host / Isotype

Mouse / IgG1

Species Reactivity

Human

Immunogen

Purified recombinant fragment of human NRCAM (AA: 1192-1255) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

WB (Western Blot)

1/500 - 1/2000

ICC (Immunocytochemistry)

1/50

ELISA

1/10000

References

1. Psychiatry Clin Neurosci. 2009 Feb;63(1):123-4.
2. Int J Neuropsychopharmacol. 2009 Feb;12(1):1-10.

Product Image

Western Blot

Figure 1: Western blot analysis using NRCAM mAb against human NRCAM recombinant protein. (Expected MW is 32.7 kDa)

Western Blot

Figure 2: Western blot analysis using NRCAM mAb against HEK293 (1) and NRCAM (AA: 1192-1255)-hIgGFc transfected HEK293 (2) cell lysate.

Immunofluorescence analysis

Figure 3: Immunofluorescence analysis of HepG2 cells using NRCAM mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

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