NR3C1 Primary Antibody

Item Information

Catalog #

Size

Price

Description

The protein encoded by this gene is a receptor for glucocorticoids that can act as both a transcription factor and as a regulator of other transcription factors. This protein can also be found in heteromeric cytoplasmic complexes along with heat shock factors and immunophilins. The protein is typically found in the cytoplasm until it binds a ligand, which induces transport into the nucleus. Mutations in this gene are a cause of glucocorticoid resistance, or cortisol, resistance. Tissue specificity: Widely expressed. In the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart.

Product Overview

Entrez GenelD

2908

Aliases

GR; GCR; GRL; GCCR; NR3C1

Clone#

6E6

Host / Isotype

Mouse / IgG1

Species Reactivity

Human

Immunogen

Purified recombinant fragment of human NR3C1 expressed in E. Coli.

Formulation

Ascitic fluid containing 0.03% sodium azide.

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

ICC (Immunocytochemistry)

1/200 - 1/1000

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1. J Clin Endocrinol Metab. 2008 Dec;93(12):4963-8.
2. Epigenetics. 2008 Mar-Apr;3(2):97-106.

Product Image

Western Blot

Figure 1: Western blot analysis using NR3C1 mouse mAb against Hela cell lysate.

Immunohistochemical analysis

Figure 2: Immunohistochemical analysis of paraffin-embedded lung cancer tissues using NR3C1 mouse mAb with DAB staining.

Immunofluorescence analysis

Figure 3: Immunofluorescence analysis of PC-2 cells using NR3C1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Flow cytometric