NQO1 Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. This protein's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Mutations in this gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of this protein has been seen in many tumors and is also associated with Alzheimer's disease (AD). Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Product Overview
Entrez GenelD
1728
Aliases
DTD; QR1; DHQU; DIA4; NMOR1; NMORI
Clone#
4D12
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human NQO1 expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1. Mol Cancer Ther. 2009 Dec;8(12):3369-78.
2. J Biol Chem. 2009 Nov 27;284(48):33233-41.
2. J Biol Chem. 2009 Nov 27;284(48):33233-41.
Product Image
Western Blot
Figure 1: Western blot analysis using NQO1 mAb against human NQO1 (AA: 134-274) recombinant protein. (Expected MW is 41.3 kDa)
Western Blot
Figure 2: Western blot analysis using NQO1 mouse mAb against A549 (1), SKNES (2), HepG2 (3), MCF-7 (4) and Hela (5) cell lysate.
Immunohistochemical analysis
Figure 3: Immunohistochemical analysis of paraffin-embedded testis tissues using NQO1 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 4: Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using NQO1 mouse mAb with DAB staining.
Flow cytometric
Figure 5: Flow cytometric analysis of NIH/3T3 cells using NQO1 mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.