NPT Primary Antibody
Item Information
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Description
Neomycin phosphotransferase II (nptII) gene is used in selection of transformed organisms. It was initially isolated from the transposon Tn5 that was present in the bacterium strain Escherichia coli K12. The gene codes for the aminoglycoside 3'-phosphotransferase (denoted aph(3')-II or NPTII) enzyme. NPTII is probably the most widely used selectable marker for plant transformation. It is also used in gene expression and regulation studies in different organisms in part because N-terminal fusions can be constructed that retain enzymatic activity. In animal cells, G418 and neomycin are used as selectable agents.
Product Overview
Clone#
4B4D1
Host / Isotype
Mouse / IgG2a
Species Reactivity
Human
Immunogen
Purified recombinant fragment of NPT expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ELISA
1/10000
References
1. Landis WG et.al Human and ecological Risk Assessment 6(5): 875-899.
2. Stewart CN et.al BioTechniques 29(4): 832-843.
3. Thomson JA et.al Journal of Food Science 66(2): 188-193.
2. Stewart CN et.al BioTechniques 29(4): 832-843.
3. Thomson JA et.al Journal of Food Science 66(2): 188-193.
Product Image
Western Blot
Figure 1: Western blot analysis using NPT mouse mAb against truncated NPT recombinant protein.
For Research Use Only. Not for use in diagnostic procedures.