NOX4 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesKOX; KOX-1; RENOX
ProductOrderN
Clone#3H2G11
Entrez GenelD50507
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human NOX4 (AA: 210-310) expressed in E. Coli.
MW67kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
210-310
Catalog#: 30754
Inquire
Contact us to order
Images
Elisa
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western Blot
Figure 2:Western blot analysis using NOX4 mAb against human NOX4 (AA: 210-310) recombinant protein. (Expected MW is 25.3 kDa)
Figure 2:Western blot analysis using NOX4 mAb against human NOX4 (AA: 210-310) recombinant protein. (Expected MW is 25.3 kDa)
Western Blot
Figure 3:Western blot analysis using NOX4 mAb against HEK293 (1) and NOX4 (AA: 210-310)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using NOX4 mAb against HEK293 (1) and NOX4 (AA: 210-310)-hIgGFc transfected HEK293 (2) cell lysate.
Immunofluorescence analysis
Figure 4:Immunofluorescence analysis of Hela cells using NOX4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 4:Immunofluorescence analysis of Hela cells using NOX4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 5:Flow cytometric analysis of Hela cells using NOX4 mouse mAb (green) and negative control (red).
Figure 5:Flow cytometric analysis of Hela cells using NOX4 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using NOX4 mouse mAb with DAB staining.
Figure 6:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using NOX4 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using NOX4 mouse mAb with DAB staining.
Figure 7:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using NOX4 mouse mAb with DAB staining.
Product Overview
DescriptionThis gene encodes a member of the NOX-family of enzymes that functions as the catalytic subunit the NADPH oxidase complex. The encoded protein is localized to non-phagocytic cells where it acts as an oxygen sensor and catalyzes the reduction of molecular oxygen to various reactive oxygen species (ROS). The ROS generated by this protein have been implicated in numerous biological functions including signal transduction, cell differentiation and tumor cell growth. A pseudogene has been identified on the other arm of chromosome 11. Alternative splicing results in multiple transcript variants.
References (references)
References (references)Stem Cells Dev. 2012 Aug 10;21(12):2212-21.
Cancer Biol Ther. 2010 Aug 1;10(3):223-31.