This gene encodes a member of the NOTCH family of proteins. Members of this Type I transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple different domain types. Notch signaling is an evolutionarily conserved intercellular signaling pathway that regulates interactions between physically adjacent cells through binding of Notch family receptors to their cognate ligands. The encoded preproprotein is proteolytically processed in the trans-Golgi network to generate two polypeptide chains that heterodimerize to form the mature cell-surface receptor. This receptor may play a role in vascular, renal and hepatic development. Mutations in this gene may be associated with schizophrenia. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed.
Purified antibody in PBS with 0.05% sodium azide
4℃; -20℃ for long term storage
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 2:Western blot analysis using NOTCH4 mAb against human NOTCH4 (AA: extra 118-357) recombinant protein. (Expected MW is 28.5 kDa)
Figure 3:Western blot analysis using NOTCH4 mAb against HEK293 (1) and NOTCH4 (AA: extra 118-357)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Immunofluorescence analysis of Hela cells using NOTCH4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 5:Flow cytometric analysis of Hela cells using NOTCH4 mouse mAb (green) and negative control (red).
Figure 6:Flow cytometric analysis of Jurkat cells using NOTCH4 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.