NME1 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesNB; AWD; NBS; GAAD; NM23; NDPKA; NDPK-A; NM23-H1; NME1
ProductOrderN
Clone#4B2
Entrez GenelD4830
FormulationAscitic fluid containing 0.03% sodium azide.
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human NME1 expressed in E. Coli.
MW17kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
27-177
Catalog#: 30051
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Images
Western Blot
Figure 1: Western blot analysis using NME1 mAb against NME1(AA: 27-177)-hIgGFc transfected HEK293 cell lysate.
Figure 1: Western blot analysis using NME1 mAb against NME1(AA: 27-177)-hIgGFc transfected HEK293 cell lysate.
Immunohistochemical analysis
Figure 2: Immunohistochemical analysis of paraffin-embedded human Placenta tissues using anti-NME1 mouse mAb
Figure 2: Immunohistochemical analysis of paraffin-embedded human Placenta tissues using anti-NME1 mouse mAb
Immunofluorescence analysis
Figure 3: Immunofluorescence analysis of Hela cells using NME1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Figure 3: Immunofluorescence analysis of Hela cells using NME1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Flow cytometric
Figure 4: Flow cytometric analysis of Jurkat cells using NME1 mouse mAb (green) and negative control (purple).
Figure 4: Flow cytometric analysis of Jurkat cells using NME1 mouse mAb (green) and negative control (purple).
Product Overview
Description

non-metastatic cells 1,protein,with a nm23 nucleoside diphosphate kinase gene family,involved in the phosphorylation of nucleoside diphosphates,with a reduced expression in tumor progression to the metastatic phenotype,mutated in agressive neuroblastoma,expressed in lung carcinoma cell lines not in normal lung,pyrimidine biosynthetic pathway. Involved in cell proliferation, differentiation and development, signal transduction, G protein-coupled receptor endocytosis, and gene expression. Required for neural development including neural patterning and cell fate determination. Has tumor metastasis-suppressive capacity.Tissue specificity: Isoform 1 is expressed in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, spleen and thymus. Expressed in lung carcinoma cell lines but not in normal lung tissues. Isoform 2 is ubiquitously expressed and its expression is also related to tumor differentiation. Isoform 3 is ubiquitously expressed.

References (references)
References (references)1. J Cell Biochem. 2009 Mar 1;106(4):666-72.
2. FEBS Lett. 2009 Sep 3;583(17):2789-92.
3. PLoS One. 2009 Nov 23;4(11):e7949.