NGFR Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesCD271; p75NTR; TNFRSF16; p75(NTR); Gp80-LNGFR; NGFR
ProductOrderN
Clone#2F1C2
Entrez GenelD4804
FormulationAscitic fluid containing 0.03% sodium azide.
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human NGFR expressed in E. Coli.
MW45kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
aa1-200
Catalog#: 20417
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Images
Western Blot
Figure 1: Western blot analysis using NGFR mouse mAb against NGFR-hIgGFc transfected HEK293 cell lysate.
Figure 1: Western blot analysis using NGFR mouse mAb against NGFR-hIgGFc transfected HEK293 cell lysate.
Immunofluorescence analysis
Figure 2:Immunofluorescence analysis of EC cells using NGFR mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Figure 2:Immunofluorescence analysis of EC cells using NGFR mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Flow cytometric
Figure 3: Flow cytometric analysis of EC cells using NGFR mouse mAb (green) and negative control (purple).
Figure 3: Flow cytometric analysis of EC cells using NGFR mouse mAb (green) and negative control (purple).
Product Overview
Description

Nerve growth factor receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain. NGFR p75 plays a central role in the regulation of cell number by apoptosis in the developing CNS. During early development, activation of NGFR p75 by NGF induces apoptotic cell death in some neuronal cells, probably through activation of the sphingomyelinase/ceramide pathway, the ICE like proteases and the JNK pathway. In rat Schwann cells, NGF binding to NGFR p75 activates NF kappaB, possibly to modulate Schwann cell migration during nerve regeneration. CD271 has recently been described as being expressed in mesenchymal stem cells (bone marrow stromal cells).

References (references)
References (references)1. J Invest Dermatol. 1992 Dec;99(6):734-42.
2. Nature. 1997 Aug 7;388(6642):548-54.
3. Exp Neurol. 2002 Nov;178(1):104-11.
4. Science. 2004 May 7;304(5672):870-5.