NFKBIA Primary Antibody
|Aliases||IKBA; MAD-3; NFKBI|
|Formulation||Purified antibody in PBS with 0.05% sodium azide|
|Immunogen||Purified recombinant fragment of human NFKBIA (AA: 150-291) expressed in E. Coli.|
|Shipping Information||This product will ship in a box containing blue ice at a temperature of 4°C. Learn More|
|ICC (Immunocytochemistry)||1/200 - 1/1000|
|IHC_P(Immunohistochemistry)||1/200 - 1/1000|
|FCM (Flow Cytometry)||1/200 - 1/400|
|WB (Western Blot)||1/500 - 1/2000|
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Figure 2:Western blot analysis using NFKBIA mAb against human NFKBIA (AA: 150-291) recombinant protein. (Expected MW is 42.4 kDa)
Figure 3:Western blot analysis using NFKBIA mAb against HEK293 (1) and NFKBIA (AA: 150-291)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Immunofluorescence analysis of Hela cells using NFKBIA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 5:Immunofluorescence analysis of MCF-7 cells using NFKBIA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 6:Flow cytometric analysis of A549 cells using NFKBIA mouse mAb (green) and negative control (red).
Figure 7:Immunohistochemical analysis of paraffin-embedded liver cancer tissues using NFKBIA mouse mAb with DAB staining.
Figure 8:Immunohistochemical analysis of paraffin-embedded brain tissues using NFKBIA mouse mAb with DAB staining.
|Description||This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease.|
|References (references)||1.Mol Cancer. 2013 Dec 11;12:160. |
2.Acta Med Okayama. 2013;67(1):19-24.