NEFM Primary Antibody

Item Information

Catalog #

Size

Price

Description

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Product Overview

Entrez GenelD

4741

Aliases

NFM; NEF3; NF-M

Clone#

1B6B12

Host / Isotype

Mouse / IgG1

Species Reactivity

Human

Immunogen

Purified recombinant fragment of human NEFM (AA: 779-916) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

4°C; -20°C for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

ICC (Immunocytochemistry)

1/50 - 1/200

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1.J Neuropathol Exp Neurol. 2004 Jul;63(7):759-74.
2.Neurosci Lett. 2003 Nov 13;351(2):125-9.

Product Image

Elisa

Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using NEFM mAb against human NEFM (AA: 779-916) recombinant protein. (Expected MW is *** kDa)

Western Blot

Figure 3:Western blot analysis using NEFM mAb against HEK293 (1) and NEFM (AA: 779-916)-hIgGFc transfected HEK293 (2) cell lysate.

Immunofluorescence analysis

Figure 4:Immunofluorescence analysis of Hela cells using NEFM mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric