NEFM Primary Antibody
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Description
Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Product Overview
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using NEFM mAb against human NEFM (AA: 779-916) recombinant protein. (Expected MW is *** kDa)
Western Blot
Figure 3:Western blot analysis using NEFM mAb against HEK293 (1) and NEFM (AA: 779-916)-hIgGFc transfected HEK293 (2) cell lysate.
Immunofluorescence analysis
Figure 4:Immunofluorescence analysis of Hela cells using NEFM mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 5:Flow cytometric analysis of Hela cells using NEFM mouse mAb (green) and negative control (red).
Flow cytometric
Figure 6:Flow cytometric analysis of Raji cells using NEFM mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.